PEPCK | PEP carboxy kinase
AS07 241 | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for plants A. comosus, M. giganteus, P. virgatum, P. vulgaris, Saccharum spp., S. alterniflora, S. patens, Z. mays
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1 : 1000 with standard ECL (WB)
|Expected | apparent MW||
73 | 78 kDa
Ananas comosus, Miscantus giganteus, Panicum virgatum, Phaseolus vulgaris, Saccharum spp. hybrid clone C91-301, Spartina alterniflora , Spartina patens, Zea mays, mice
Brassica napus, Chromera velia, Cucumis sativus, Flaveria sp., Lycopersicon esculentum, Medicago sativa, Oryza sativa, Panicum maximum, Urochloa panicoides, Zoysia japonica , Zea mays
|Not reactive in||
Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.
|Selected references||Aragón et al. (2013). The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles. Plant Cell Rep. Aug 20. (Ananas comosus, western blot detection following 2D gel electrophoresis)|
20 µg of total protein from (1) Arabidopsis thaliana total cell extracted with Protein Extration Buffer, PEB (AS08 300), (2) Phaseolus vulgaris total cell, extracted with PEB, (3) Zea mays total cell extracted with PEB, (4) Miscanthus giganteus total cell extracted with PEB, (5) Panicum virgatum total cell extracted with PEB, (6) Spartina alterniflora total cell extracted with PEB, (7) Spartina patens total cell extracted with PEB, were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).
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