PsaB | PSI-B core subunit of photosystem I

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AS10 695 | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for higher plants, algae, cyanobacteria


62 st
Item No:
AS10 695

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product information

Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaB is a core protein of PSI complex. Synonymes: Photosystem I P700 chlorophyll a apoprotein A2, PSI-B


KLH-conjugated synthetic peptide derived from known PsaB sequences including Arabidopsis thaliana P56767, AtCg00340

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 172 | anti-PsaA rabbit antibody
AS10 695-50 | PsaB | PSI-B core subunit of photosystem I (50 µl)
collection of antibodies to PSI proteins

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

82.7 | 55-60 kDa

Confirmed reactivity

Arabidopsis thaliana, Bryopsis corticulans, Hordeum vulgare, Nicotiana tabacum, Pisum sativum, Solanum lycopersicum, Synechococcus sp. PCC7942, Synechocystis sp. PCC 6803

Predicted reactivity

algae, Aloysia triphylla, Beta vulgaris, Borago officinalis, Brachypodium distachyon, Cercidiphyllum japonicum, Citrus x limon, cyanobacteria, Exbucklandia populnea, Gunnera manicata, Kalanchoe laciniata, Lagenaria siceraria, Lippia origanoides, Lippia alba, Indocalamus sinicus, Morus notabilis, Medicago truncatula, Monsonia emarginata, Mytilaria laosensis, Geranium endressii, Glycine max, Glycine soja, Lotus japonicus, Oryza sativa, Pandanus utilis, Panax ginseng, Parnassia laxmannii , Pelargonium cotyledonis, Pennisetum americanum, Phyla dulcis, Phaseolus pachyrrhizoides, Phaseolus lunatus, Phaseolus vulgarisPinus thunbergiiPopulus trichocarpa, Ribes fasciculatum, Rhodoleia championii, Rhyticaryum macrocarpum, Salvia miltiorrhiza, Setaria italica, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Vigna angularis, Vitis vinifera, Zea mays

Not reactive in

Chlamydomonas reinhardtii

Additional information

to be added when available

Selected references Suorsa et al. (2015). Light acclimation involves dynamic re-organisation of the pigment-protein megacomplexes in non-appressed thylakoid domains. Plant J. 2015 Aug 29. doi: 10.1111/tpj.13004. Grieco et al. (2015). Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery - including both photosystems II and I. Biochim Biophys Acta. 2015 Jun-Jul;1847(6-7):607-19. doi: 10.1016/j.bbabio.2015.03.004. Epub 2015 Apr 3.
Subramanyam et al. (2014). Structural and functional changes of PSI-LHCI supercomplexes of Chlamydomonas reinhardtii cells grown under high salt conditions. Planta. 2010 Mar;231(4):913-22.
Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.
Mustila et al. (2014). The bacterial-type [4Fe–4S] ferredoxin 7 has a regulatory function under photooxidative stress conditions in the cyanobacterium Synechocystis sp. PCC 6803. BBA- Bioenergetics, April 2014.

application example

5 µg of total protein from (1) Arabidopsis thaliana leaf extract, (2) Synechococcus sp. PCC 7942 , (3) Hordeum vulgare leaf extract, (4) Physcomitrella patens, (5) Pisum sativum, (6) Zea may were extracted with Agrisera Protein Extraction Buffer PEB and separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose OSMONICS. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.


western blot using anti-PsaB antibodies

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