PsbD | D2 protein of PSII

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AS06 146  |  clonality: polyclonal  |  host: rabbit  |  reactivity: [global antibody] for A.thaliana, H. vulgare, L. corniculatus, N. tabacum, O. sativa, P. sativum, P. vulgaris, T. pratense, U. prolifera, C. reinhardtii, Synechococcus sp. PCC 7942, Anabaena 7120, diatoms


47 st
Item No:
AS06 146

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product information

D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.


KLH-comjugated synthetic peptide derived from the C-terminal of known PsbD sequences including Arabidopsis pumila A4QJS8 , Hordeum vulgare P11849, Chlamydomonas reinhardtii P06007, Synechococcus sp. PCC 7002 P20898

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS09 146S | PsbD | D2 protein of PSII protein standard for quantitation and as a positive control
AS06 146PRE | PsbD | D2 protein of PSII, pre-immune serum
AS05 084 | anti-PsbA (D1) protein of PSII, C-terminal
collection of antibodies to PSII proteins
recommended secondary antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

The peptide used to elicit this antibody has a perfect conservation across all full-length PsbD sequences from higher plants, lower
plants, cyanobacteria and unicellular algae except:   -minor substitutions in some Prochlorococcus & Dinoflagellate sequences. The antibody should still work against these taxa, but it has not been tested yet.  This antibody does not detect PsbA protein (D1).

application information
Recommended dilution

1: 5000 with standard ECL (WB)

Expected | apparent MW

39.4 | 28-30 kDa

Confirmed reactivity

Arabidopsis thaliana, Horderum vulgare, Chlamydomonas reinhardtii, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Trifolium pratense, Sinapsis alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Anabaena 7120, Ditylum brightwellii, Thalassiosira guillardii, Thalassiosira pseudonana,
Ulva prolifera

Predicted reactivity

Cucumis sativa, Glycine max, Glycine Soja, Ricinus communis, Panax ginseng, Pinus thunbergii, Physcomitrella patens, Populus trichocarp, Solanum tuberosumSpinacia oleracea, Triticum aestivum, Vitis vinifera, Zea mays, Crocosphaera watsonii, Fischerella sp., Leiosporoceros dussii, Centrolepsis monogyna, Microcystis aeruginosa, Petermannia cirrosa, Vitrella brassicaformis, Chromera velia, Utricularia alpina, Aegilops tauschii, Nannochloropsis

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

There is a confirmed cross-reaction with TLA1 protein in Chlamydomonas reinhardtii.

For samples with a very low PSII content theremight be detection problems independent of the antibody. PSII proteins can vary in level depending upon liquid culture conditions. When the cells are in a stationary phase PSII content can drop to a very low level.

Selected references Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. Mazur et al. BMC Plant Biology (2016) 16:191.
Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015.
Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29.
Huang et al. (2015). Rubisco accumulation is important for the greening of the fln2-4 mutant in Arabidopsis. Volume 236, July 2015, Pages 185–194.
Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741.
Malnoë et al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21.

application example

2 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extraction Buffer, PEB (AS08 300), (2) Hordeum vulgare leaf extracted with PEB, (3) Chlamydomonas reinhardtii total cell extracted with PEB, (4) Synechococcus sp. 7942 total cell extracted with PEB, (5) Anabaena sp. total cell extracted with PEB were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 3 seconds.


 western blot using anti-PsbD antibodies

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