L13-1 | 60S ribosomal protein L13-1

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AS13 2650 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Hordeum vulgare


44 st
Item No:
AS13 2650

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product information

L13 protein is localized in the cytoplasm. It belongs to the ribosomal protein L13e family. Alternative name: protein BBC1 homolog.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana UniProt: P41127, TAIR: At3g49010

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Quantity 200 ĩg
Reconstitution For reconstitution please add 100 ĩl For reconstitution

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 2500 with standard ECL (WB)

Expected | apparent MW

23.7 | 29 kDa (Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare

Predicted reactivity

dicots including: Brassica napus, Nicotiana tabacum, Ricinus communis, Vitis vinifera, monocots including: Oryza sativa, Sorghum bicolor, Zea mays, trees: Picea excelsa, Populus balsamifera, algae: Chlamydomonas reinhardii,

Not reactive in


Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Selected references

to be added when available, antibody released in October 2013.

application example

western blot using anti-L13 antibodies
10 µg of total protein from Arabidopsis thaliana (1) and Hordeum vulgare (2) leaf, extracted with Protein Extraction Buffer PEB (AS08 300), were supplemented with 50 mM DTT and heat at 70°C for 5 min and keep on ice before loading. Protein separation was done using NuPage 4-12% Tris-Bis gel (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2-2.5 % RPN2125 (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2500 in blocking reagent for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:25 000 in TBS-T for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with TMA-6 (Lumigen) detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 2 minutes.
Note: western blot detection pattern can be different if other type of samples is used, for example membrane fraction.

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