RPS12 | Ribosomal protein S12 (chloroplastic)

345 €

AS12 2114 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii


11 st
Item No:
AS12 2114

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product information

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.


Recombinant full length RPS12 of Chlamydomonas reinhardtii, P14149, expressed in E.coli

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Plant and algal protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1 : 10 000 with standard ECL (WB)

Expected | apparent MW

14.6 kDa

Confirmed reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii

Predicted reactivity Spinacia oleracea, Pinus sp., Physcomitrella patens, Synechocystis sp. PCC6803, Synechococcus elongatus PCC 7942, Chlorella vulgaris, Dunaliella salina, Scenedesmus obliquus,Volvox carteri (green alga), Oltmannsiellopsis viridis (marine flagellate), Prochlorococcus marinus, bacteria
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references

Ramundo et al. (2013). Repression of Essential Chloroplast Genes Reveals New Signaling Pathways and Regulatory Feedback Loops in Chlamydomonas. The Plant Cell.

application example

western blot using anti-RPS12 antibodies

10 µg of total protein from Chlamydomonas reinhardtii extracted with standard lysis buffer (Tris-HCl pH 6.8 50mM, 10mM EDTA, 2% SDS, Sigma protease inhibitors)  were separated on 15 % SDS-PAGE and blotted to nitrocellulose membrane using a wet transfer cell. Blot was blocked in TBS-T containing 5% non-fat dry milk  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T containing 5% non-fat dry milk  for 1h at RT with agitation. The antibody solution was decanted and the blot was washed  3 times for 15 min  in TBS-T containing 1% non-fat dry milk with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Promega) diluted to 1:0 000 in TBS-T containing 1% non-fat dry milk  for 1h at RT with agitation. The blot was washed as above  and developed for 1 min with ECL according to the manufacturers instructions. Exposure time was 30 seconds.

Courtesy of Dr. Silvia Ramundo, University of Geneva, Switzerland

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