RPS12 | Ribosomal protein S12 (chloroplastic)
AS12 2114 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii
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1 : 10 000 with standard ECL (WB)
|Expected | apparent MW||
Arabidopsis thaliana, Chlamydomonas reinhardtii
|Predicted reactivity||Spinacia oleracea, Pinus sp., Physcomitrella patens, Synechocystis sp. PCC6803, Synechococcus elongatus PCC 7942, Chlorella vulgaris, Dunaliella salina, Scenedesmus obliquus,Volvox carteri (green alga), Oltmannsiellopsis viridis (marine flagellate), Prochlorococcus marinus, bacteria|
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
to be added when available
Ramundo et al. (2013). Repression of Essential Chloroplast Genes Reveals New Signaling Pathways and Regulatory Feedback Loops in Chlamydomonas. The Plant Cell.
10 µg of total protein from Chlamydomonas reinhardtii extracted with standard lysis buffer (Tris-HCl pH 6.8 50mM, 10mM EDTA, 2% SDS, Sigma protease inhibitors) were separated on 15 % SDS-PAGE and blotted to nitrocellulose membrane using a wet transfer cell. Blot was blocked in TBS-T containing 5% non-fat dry milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T containing 5% non-fat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was washed 3 times for 15 min in TBS-T containing 1% non-fat dry milk with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Promega) diluted to 1:0 000 in TBS-T containing 1% non-fat dry milk for 1h at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturers instructions. Exposure time was 30 seconds.
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