Sec21p | gamma subunit, COP vesicles

350 €

AS08 327 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Zea mays | cellular [compartment marker] of Golgi in immunolocalization and of COP1 in western blot


27 st
Item No:
AS08 327

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product information

Sec21p is a constituent of the COPI vesicle coatomer.


GST fusion of a part of recombinant Sec21 of Arabidopsis thaliana Q0WW26, At4g34450

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunofluorescence (IF)
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AS08 325 | anti-Arf1 | ADP-ribosylation factor 1, Golgi marker, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1000 with standard ECL (WB), 1: 1000 (IF)

Expected | apparent MW

98 kDa

Confirmed reactivity

Arabidopsis thaliana, Zea mays

Predicted reactivity

Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Coffea canephora, Eutrema salsugineum, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Medicago truncatula, Oryza sativa, Populus trichocarpaPrunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays,

Not reactive in

Nicotiana tabacum, Microsporidia sp.

Additional information

This antibody can be used as a Golgi marker in immunolocalization and as a marker of COP1 in Western blot.

References describing immunolocalization (IF) and (IG) studies:

Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36.
Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.

Selected references Wattelet-Boyer et al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788.
Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.
Tanaka et al. (2013). Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana. PLoS Genet. May;9(5). (immunolocalization). 
Hopff et al. (2013). The plasma membrane proteome of maize roots grown under low and high iron conditions. J Proteomics Jan 24.
Pimpl et al (2000). In situ localization and in vitro induction of plant COPI-coated vesicles. Plant Cell. 2000 Nov;12(11):2219-36.

application example

50 µg of total protein from (1) Nicotiana tabacum protoplast total protein, (2) Arabidopsis thaliana protoplast soluble protein, (3) Arabidopsis thaliana protoplast total protein were separated on 10 % SDS-PAGE and blotted 2h to nitrocellulose (Semi-dry, 200mA). Filters were blocked over night with 5% low-fat milk powder in TBS and probed with anti-Sec21p antibodies (AS08 327, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP) in TBS-Tween. Signal was detected with standard ECL andexposure time for this image was 1 minute.

Protoplasts were extracted in 50mM Tris, 10 mM EDTA and Triton X100, 0.02%.

Western blot using anti-Sec21p



Immunofluorescence labelling of rabbit anti-SEC21 (gamma subunit of COP vesicles; red) in 5-day-old root epidermal cells of Arabidopsis thaliana expressing ER-mGFP5-HDEL (ER marker; green). The antibody was diluted 1:1000 and the secondary antibody, donkey anti-rabbit CY5-coupled (Jackson ImmunoResearch) was diluted 1:300. The nuclei were stained with DAPI (blue).

Courtesy of Dr. Anna Gustavsson and Dr. Markus Grebe

immunofluorescence using anti-Sec21 antibodies

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