Ca2+-ATPase | Calmodulin-stimulated calcium-ATPase
AS09 486 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Brassica oleracea, Raphanus sativus
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1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
|Expected | apparent MW||
111 | 110 kDa (Raphanus sativus)
Arabidopsis thaliana, Brassica oleracea, Raphanus sativus
dicots including: Vitis vinifera, monocots including: Oryza sativa, Triticum aestivum, trees: Populus balsamifera, moss: Physcomitrella patens
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Manufactured by Operon Biotechnologies.
to be added when available
1 µg and 10 µg of crude membrane fraction/lane from Raphabus sativa L. Tokinashi-daikon were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-Ca2+ ATPase antibodies (AS09 486, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
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