PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) aquaporins

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AS09 487 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, J. curcas, P. nigra, P. trichocarpa, R. sativus


30 st
Item No:
AS09 487

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product information

PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B,


synthetic peptide conserved in Arabidopsis thaliana: PIP1;1 P61837,At3g61430 PIP1;2 Q06611,At2g45960 PIP1;3 Q08733,At1g01620 , PIP1;4 Q39196, At4g00430, PIP1;5 Q8LAA6 At4g23400

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies to plasma membrane proteins

AS09 488 | anti-PIP2;1 | aquaporin PIP2;1

AS09 489 | anti-PIP1;1, PIP1;2, PIP1;3 | aquaporins

AS09 490 | anti-PIP2;2 | plasma membrane aquaporin 2b

AS09 491 | anti-PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3

Plant protein extraction buffer

Secondary antibodies

Additional information

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol for plasma membrane isolation can be found here.

application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

30.68 | 28 kDa

Confirmed reactivity

Arabidopsis thaliana, Brassica sp., Jatropha curcas L. cv. "Biji Jarak ", Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea

Predicted reactivity

dicots including: Brassica sp., Vicia faba, monocots: Hordeum vulgare, Oryza sativa, Triticum aestivum, trees: Juglans regia, Populus tremula,

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.



Selected references Lopez et al. (2013). Aquaporins And Leaf Hydraulics, Poplar Sheds New Light. Plant Cell Physiol. Sep 20.

et al. (2013). Two aquaporins of Jatropha are regulated differentially during drought stress and subsequent recovery. J Plant Physiol. March 25.

application information

western blot using anti-PIP global antibodies

10 µg of Arabidopsis thaliana tonoplast fraction (1), Thellungiella salsuginea tonoplast fraction (2), Mesembryanthemum crystallinum tonoplast fraction (3), Nicotiana tabacum tonoplast fraction (4), Arabidopsis thaliana plasma membrane fraction (5), Thellungiella salsuginea plasma membrane fraction (6), Mesembryanthemum crystallinum plasma membrane fraction (7), Arabidopsis halleri microsome fraction (8), Brassica sp. microsomal fraction (9) were extracted with Protein Extraction Buffer PEB (AS08 300). Samples were diluted with 1X sample buffer (NuPAGE LDS sample buffer (Invitrogen) supplemented with 50 mM DTT and heat at 70°C for 5 min and keept on ice before loading. Protein samples were separated on 4-12% Bolt Plus gels,  LDS-PAGE and blotted for 70 minutes to PVDF using tank transfer. Blots were blocked immediately following transfer in 2% blocking reagent (GE RPN 2125; Healthcare) or 5% non-fat milk dissolved in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 (in blocking reagent) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed 1x15 min and 3x5 min with TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602,Agrisera) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above. The blot was developed for 5 min with Luminata detection reagent according the manufacturers instructions (Millipore). Images of the blots were obtained using a CCD imager (VersaDoc MP 4000) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.

Courtesy of Dr. Rosario Vera, UNAM, Mexico

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