PIP2-1-7 | Plasma membrane aquaporin isoforms 1-7 (C-terminal)

361 €

AS12 2110 | clonality: polyclonal | host: rabbit | reactivity: L. sativa, P. sativum, S. lycopersicum, Z. mays


3 st
Item No:
AS12 2110

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product information

Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane. Alternative names:  plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a


KLH-conjugated synthetic peptide derived from Zea mays PIP2-7 C-terminal, Q9ATM4, conserved also in Zea mays PIP2-1, UniProt: Q84RL7,  PIP2-2, UniProt: Q9ATM8, PIP2-3 (80 % conservation) UniProt: Q9ATM7, PIP2-4 (80 % conservation) UniProt: Q9ATM6, PIP2-5 (70 % conservation) UniProt: Q9XF58,  PIP2-6 (50 % conservation) UniProt: Q9ATM5

Host Rabbit
Clonality Polyclonal
Purity Serum
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS09 488 | PIP2;1 | aquaporin PIP2;1

AS09 490 | PIP2;2 | plasma membrane aquaporin 2b

AS09 491 | PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3

Plant protein extraction buffer

Secondary antibodies

Additional information Protocol for isolation of plant plasma membrane proteins can be found here.

This antibody has a potential to work in immunolocalization studies, as it is recognizing C-terminal part of the sequence.

application information
Recommended dilution

1: 3000 with standard ECL (WB)

Expected | apparent MW

30.7 | 30 kDa (Zea mays)

Confirmed reactivity

Lactuca sativa, Pisum sativum, Solanum lycopersicum, Zea mays

Predicted reactivity

Arabidopsis thaliana, Brassica oleracea, Cicer arietinum, Coffea arabica, Cucumis sativus, Fragaria chiloensis, Glycine max, Medicago trunculata, Mimosa pudica, Nicotiana tabacum, Olea europaea, Phaseolus vulgaris, Pisum sativum, Pyrus communis, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum urartu, Vitis vinifera,monocots: Hordeum vulgare, Oryza sativa, Triticum aestivum,  trees: Picea mariana, Populus trichocarpa

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information detection pattern consists of di and monomer of PIP2-7
Selected references

to be added when available, antibody released in May 2012.

application example

western blot detection of PIP2-7 in maize, bean

10 µg of total protein from Zea mays roots (1), Phaseolus vulgaris leaves (2) or roots (3) extracted with a mixture of 250 mM sorbitol, 50 mM Tris–HCl (pH 8), 2 mM EDTA, and protease inhibitors [1 mM phenylmethylsulfonyl Xuoride, 1 mg ml-1 each of leupeptin, aprotinin, antipain, chymostatin, and pepstatin were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% milk in TBS-T for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3.000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed four times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:30 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 seconds.

Courtesy of Dr. Ricardo Aroca, CSIC, Spain

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