MRP1, ABCC1 | ABC transporter C family member 1

440 €

AS09 472 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


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Item No:
AS09 472

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product information

MRP1 (EC= is a pump for glutathione S-conjugates. Alternative names: ABC transporter ABCC.1, multidrug resistance-associated protein 1, glutathione S-conjugate-transporting ATPase 1, ATP-energized glutathione S-conjugate pump 1


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MRP1,Q9C8G9, At1g30400

Host Rabbit
Clonality Polyclonal
Purity Serum
Quantity 100 ĩl

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), western blot (WB)
Related products

collection of antibodies to tonoplast proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol for plant tonoplast membrane preparation is available here.

application information
Recommended dilution

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

Expected | apparent MW

181.9 |180 kDa (Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

dicots including: Vitis vinifera, monocots including: Oryza sativa, Triticum aestivum, trees: Populus balsamifera, moss: Physcomitrella patens

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

Selected references

Geisler et al., (2004). Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters.Mol. Biol. Cell. 15, 3393-3405.

application example



1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AtMTP4 antibodies (AS09 472, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.


western blot using anti-AtABCC1 antibodies

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