Ligand-gated ion channel 1.3

440 €

AS09 475 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Raphanus sativus


1 st
Item No:
AS09 475

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product information

Glutamate receptor 1.3 might function as non-selective cation channel.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9FH75, At5g48410

Host Rabbit
Clonality Polyclonal
Purity Serum
Quantity 100 ĩl

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), western blot (WB)
Related products

collection of antibodies to tonoplast proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

This protein is expressed in low levels in plant tissues.

Protocol for preparation of plant vacuolar membranes can be found here.

application information
Recommended dilution

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

Expected | apparent MW

97 |100 kDa

Confirmed reactivity

Arabidopsis thaliana, Raphanus sativus

Predicted reactivity

to be determined

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

Selected references

to be added when available

application example



5 µg and 10 µg of vacuolar membrane fraction/lane from Raphanus sativus were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-ligand-gated ion channel antibodies (AS09 475, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.


western blot detection using anti-ligand-gated ion channel antibodies

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