Na+/H+ antiporter, sodium/hydrogen exchanger
AS09 484 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1: 1000 with standard ECL (WB), 1: 8000 (ELISA)
|Expected | apparent MW||
59.5 | 45 kDa
Arabidopsis thaliana, Kandelia obovata
dictos including: Gossypium hirsutum, Ricinus communis, Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Populus euphratica
|Not reactive in||
mangrove plants, sp. Avicennia, Nicotiana benthamiana
Chen et al. (2013). Nitric Oxide Mediates Root K+/Na+ Balance in a Mangrove Plant, Kandelia obovata, by Enhancing the Expression of AKT1-Type K+ Channel and Na+/H+ Antiporter under High Salinity. 8(8): e71543. doi:10.1371/journal.pone.0071543.
1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana (left panel)and1,5 and 10 µg of crude membrane fraction/lane Raphanus sativus L.(right panel)were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-NHX antibodies (AS09 484, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
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