TIP2;1 | tonoplast intrinistic protein 2-1

440 €

AS09 509 | clonality: polyclonal | host: rabbit | reactivity: Oryza sativa


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Item No:
AS09 509

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product information

TIP2-1 belongs to aquaporin protein family and facilitates transport of water and small neutral solutes across tonoplast membrane.


KLH-cionjugated peptide derived from Oryza sativa TIP2-1 Q7XA61

Host Rabbit
Clonality Polyclonal
Purity Serum
Quantity 100 ĩl

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), western blot (WB)
Related products

collection of antibodies to tonoplast proteins

AS09 492 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 494 | anti-TIP2;1 (delta) | Delta-VM23

AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1

AS09 510 | anti-TIP2;2 | tonoplast intrinistic protein 2-2

AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)

Plant protein extraction buffer

Secondary antibodies

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Vacuolar membrane protocol is available here.

application information
Recommended dilution

1: 8000 (ELISA), 1: 2000 with standard ECL (WB), immunolocalization (IL)

Expected | apparent MW

24.8 | 20-24 kDa

Confirmed reactivity

Oryza sativa, Populus trichocarpa x deltoides, clone H11-11

Predicted reactivity

Gladiolus hybrid, Hordeum vulgare, Triticum aestivum, Zea mays

Not reactive in

Arabidopsis thaliana

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

Selected references

Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39. 

application example



1.5 µg of crude membrane fraction/lane from Oryza sativa  L. cv. Akitakomachi leaf blade and root were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-TIP2;1 antibodies (AS09 509, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.


Peptide used to elicti this antibody has been added to primary antibody incubation (right panel, + +) and therefore specific signal has been depleted in this neutralization/immunocompetition assay.


  western blot using anti-TIP2;1 antibodies

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