V-ATPase, A | vacuolar H+-ATPase subunit A
AS09 467 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
|Expected | apparent MW||
68.8 | 70 kDa (Arabidopsis thaliana)
dicots including: Brassica napus, Cucumis sativus, Gossypium mexicanum, Solanum lycopersicon, Phaseolus aureus, monocots including: Hordeum vulgare, Oryza sativa, Triticum aestivum, Zea mays, trees: Populus balsamifera, moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii, Ostreococcus lucimarinus
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Manufactured by Operon Biotechnologies.
Yoshihiro et al. (2006) Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension-cultured cells. Cells. Biosci.Biotechnol. Biochem. 70: 980-987.
1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-V-ATPase subunit A antibodies (AS09 467, 1:2000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
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