V-ATPase, c | vacuolar H+-ATPase, subunit c (16 kDa)
AS09 468 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Mesembryanthemum crystallinum
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1: 1000 with standard ECL (WB)
|Expected | apparent MW||
16 | 16 kDa (Arabidopsis thaliana)
Arabidopsis thaliana, Mesembryanthemum crystallinum
dictos including: Cucumis sativus, Gossypium mexicanum, Phaseolus aureus, Raphanus sativus, Rcicinus communis, monocots including: Oryza sativa, Triticum aestivum, Zea mays, trees: Picea sitchensis, Populus trichocarpa
|Not reactive in||
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
|Selected references||to be added when available, antibody released in June 2014.|
Following samples were analyzed: MW markers (1), Arabidopsis thaliana tonoplast (2), Arabidopsis thaliana plasma membrane (3), Arabidopsis thaliana microsomes (4), Arabidopsis thaliana total protein (5), Mesembryanthemum crystallinum tonoplast (6), Mesembryanthemum crystallinum plasma membrane (7), Mesembryanthemum crystallinum microsomes (8), Nicotiana tabacum microsomes (9), Brassica napus microsomes (10). 15 µg of the indicated protein, extracted according to Vera-Estrella et al. (2012) was separated on 12% SDS-PAGE and blotted 1.15h to PVDF using tank transfer. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 O/N at RT with agitation. The antibody solution was decanted and the blot was washed 3X for 15 min min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602 ) diluted to 1:50 000 in for 2h at RT with agitation. The blot was washed as above and developed using the Millipore Luminata Crescendo substrate using the LiCOR c-DIGIT personal imager.
Courtesy Dr. Bronwyn Barkla. UNAM, Mexico
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