AtpA | alpha subunit of ATP synthase

345 €

AS08 304  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A.thaliana, H. modesticaldum, Z.mays


4 st
Item No:
AS08 304

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product information

ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.


Recombinant maize chloroplast AtpA P05022

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunoprecipitation (IP), Western blot (WB)
Related products

AS03 030 | anti-ATP synthase subunit beta hen antibody

AS08 312 | anti-ATP synthase subunit gamma antibody

AS05 071 | anti-ATP synthase subunit c antibody

AS08 370 | anti-ATP synthase whole enzyme

Additional information

sequence of protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757

application information
Recommended dilution

1: 5 000 with standard ECL (WB)

Expected | apparent MW

55 kDa

Confirmed reactivity

Arabidopsis thaliana, Capsicum annuum, Heliobacterium modesticaldum, Zea mays

Predicted reactivity

established to react with dicot and monocot chloroplast AtpA; may cross-react with mitochondrial AtpA; algae, cyanobacteria

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370. Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.

application example


Western blot
Lane 1: Arabidopsis thaliana total leaf protein extract;
Lane 2: Zea mays total leaf protein extract
5 µg of total protein were loaded per each lane.
Experimental conditions:
Proteins were separated in a 12% or 5-15% gradient gel following by a transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies. Manufacturer of secondary antibodies: Bio-Rad
AS08 304 western blot picture.jpg

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