AtpH | ATP synthase subunit c

345 €

AS05 071  clonality: polyclonal  |  host: rabbit  |  reactivity: A.thaliana, S.oleracea, N.benthamina. T. elongatus


29 st
Item No:
AS05 071

Info: Product suggestions Add review
product information

F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.


purified c subunit from Spinacia oleracea P69447

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

AS08 370 | anti-ATP synthase whole enzyme

AS08 304 | anti-ATP synthase subunit alpha

AS05 085 | anti-ATP synthase subunit beta

AS08 312 | anti-ATP synthase subunit gamma antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1: 1000 - 1: 10 000 with standard ECL (WB)

Expected | apparent MW

8 kDa (for Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus

Predicted reactivity

dicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing Oryza sativaZea mays, trees: Populus alba, Pinus thunbergii, moss: Physcomitrella patens,  green algae

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

please note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur

Selected references

Lawrence et al. (2010). Recombinant production and purification of the subunit c of chloroplast ATP synthase. Protein Expression and Purification 76: 15-24.

application example

AS05 071 western picture.jpg

0.7 μg of   purified ATP-synthase complex from Spinacia oleracea (CF0F1) (1), 15.3 µg of purified ATP-synthase complex (CF0F1) from Nicotiana benthamiana (2) and 48.6 µg of Thermosynechococcus elongatus thylakoid preparation (3) were separated on 12% polyacrylamide gel and blotted on PVDF membrane. Filters where blocked (0.5h), incubated with 1: 1000 anti-AtpH antibodies (1h), followed by incubation with 1: 5 000 secondary anti-rabbit antibody (1.25h), coupled to HRP and visualized with standard ECL.

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