AtpF | CF0I subunit of ATP synthase

350 €
AS10 1604 | clonality: polyclonal | host: rabbit | reactivity:A. thaliana, S. oleracea, Ch. reinhardtii


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Item No:
AS10 1604

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product information
Background The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Immunogen isolated CF0I subunit of the chloroplast ATP synthase complex
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Liquid
Quantity 100 µl
Storage Short term 4°C. Long term -20°. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide as preservative.
Tested applications western blot (WB)
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Secondary antibodies

Additional information

This product can be sold containing proClin if requested.

application information
Recommended dilution 1: 5000 with standard ECL (WB)
Expected | apparent MW

21 kDa

Confirmed reactivity

Arabidopsis thaliana, Spinacia oleracea, Chlamydomonas reinhardtii

Predicted reactivity higher plants
Not reactive in no confirmed exceptions from predicted reactivity known in the moment
Additional information to be added when available
Selected references Grieco et al. (2015). Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery - including both photosystems II and I. Biochim Biophys Acta. 2015 Jun-Jul;1847(6-7):607-19. doi: 10.1016/j.bbabio.2015.03.004. Epub 2015 Apr 3.

Yap at al. (2015). AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5 and also promotes atpF splicing in Arabidopsis and rice. Plant J. 2015 Jan 13. doi: 10.1111/tpj.12756.

application example

western blot using anti-AtpF antibodies

20 µg of chloroplast fraction from Arabidopsis thaliana (1) and Spinacia oleracea (2)  were separated on 12  % SDS-PAGE  and blotted 1h to PVDF. Blots were blocked with 2 % non-fat milk powder in 1xTBS-T  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions.

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