AtpG | ATPsynthase subunit II b', chloroplastic

345 €

AS09 457 | clonality:polyclonal | host:rabbit | reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii


15 st
Item No:
AS09 457

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product information

ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.

Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'


KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8, At4g32260 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

AS08 370 | anti-ATP synthase, whole enzyme

AS08 304 | anti-ATP synthase subunit alpha antibody

AS03 030 | anti-ATP synthase subunit beta hen antibody

AS05 071 | anti-ATP synthase subunit c antibody

AS08 312 | anti-AtpC | gamma subunit of ATP synthase

AS07 260 | H+ATPase | plasma membrane H+ATPase

Additional information
application information
Recommended dilution

1: 10 000 (WB)

Expected | apparent MW

22.4 kDa (Chlamydomonas reinhardtii) 15.9 kDa (Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii

Predicted reactivity

dicots including: Sorghum bicolor, monocots including: Oryza sativa, Zea mays, algae Volvox carteri

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Antibody reactivity for Arabidopsis thaliana has been confirmed on membrane fraction.

Selected references

to be added when available. Antibodies released in November 2009.

application example

10 ug of chlorophyll/well of Chlamydomonas reinhardtii total cell extract (1), Chlamydomonas reinhardtii subunit II deletion mutant thylakoid membrane fraction (2), Arabidospsis thaliana thylakoid membrane fraction (3), were separated on 12-18% acrylamide-8M urea gel and blotted to nitrocellulose membrane. Filters were blocked 1 h with 5% dry milk in 1 x PBS and probed with anti-ATP synthase subunit gamma antibody (AS09 457, 1: 10 000, 1h) and secondary HRP-conjugated anti-rabbit antibody (1: 10 000, 1 h) in 1 x PBS containing 5% dry milk. All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare), exposure time was 1 min.

Arabidopsis membrane preparation has been done according to Lezhneva et al. (2008) A novel pathway of cytochrome c biogenesis is involved in the assembly of the cytochrome b6f complex in arabidopsis chloroplasts. J Biol. Chem., 283:24608-24616 and Chlamydomonas membranes were prepared according to Chua & Bennoun (1975) Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. PNAS 72:2175-2179

Courtesy Dr. Yves Choquet


western blot detection using anti-AtpG subunit II antibodies

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