FDX1 | Ferredoxin

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AS06 121  | clonality: polyclonal  | host: rabbit  | reactivity: A. thaliana, H. vulgare,  P. strobus, S. oleracea, Ch. reinhardtii 


19 st
Item No:
AS06 121

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product information

Ferredoxins are acidic, low molecular weight, soluble iron-sulfur proteins found in various organisms. Iron-sulfur proteins are defined as proteins carrying iron-sulfur cluster(s) in which the iron is at least partially coordinated by sulfur. The protein acts as multifunctional electron carriers in diverse redox systems. The chloroplast ferredoxin is involved in both cyclic and non-cyclic photophosphorylation reactions of photosynthesis and other reductive reactions in the chloroplast.


Native ferredoxin purified from Spinacia oleracea.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies to other proteins involved in electron transfer

Additional information

In Arabidopsis thaliana leaf extracts there is a strong cross-reactivity at 20 kDa.

application information
Recommended dilution

1: 1000 with alkaline phosphatase or standard ECL (WB)

Expected | apparent MW

10 (Spinacia oleracea); 16.7 (Arabidopsis thaliana), 13.7 (Chlamydomonas reinhardtii)

Confirmed reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Pinus strobus, Spinacia oleracea

Predicted reactivity

dicotyl plants including Nicotiana tabacum

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Load per well: 20-40  µg/lane required for Arabidopsis thaliana, 2-10 µg for other species.

This product can be sold containing proclin if requested.

Selected references Higuchi et al. (2011). Modulation of macronutrient metabolism in barley leaves under iron-deficient condition. Soil Sc and Plant Nutr. 57 (2): 233-247.

application example

western blot detection using anti-ferredoxin antibodies

20 or 40 µg of total protein from Arabidopsis thaliana (WT) leafs or ferredoxin mutant fd2 were separated on 15 %  acrylamide gel with 6 M urea. Filters were blotted on PVDF, blocked (1 h) with 5 % milk, incubated with 1: 1000 anti-ferredoxin (over night in 4ºC) in 1 % milk/TBS-T) followed by incubation with 1: 10000 secondary antibody (2 h) coupled to HRP and visualization with standard ECL. 

western blot using anti-Fdx antibodies on Chlamydomonas extracts
20 or 40 µg of total protein from Arabidopsis thaliana (WT) leafs or ferredoxin mutant fd2 were separated on 15 %  acrylamide gel with 6 M urea. Filters were blotted on PVDF,5 µg of soluble protein extracts from Chlamydomonas reinhardtii strain CC-4532 were separated on 15 % SDS-PAGE and blotted at 4 W for 30 min to nitrocellulose membrane (0.1 µm pore size) using semi-dry transfer. The blot was blocked with 1 % non-fat dry milk overnight at room temperature (RT) with agitation. The blot was incubated in the primary antibody solution at a dilution of 1: 10 000 in PBS-T+1% milk for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. The blot was incubated in secondary antibody solution (anti-rabbit IgG-AP conjugated) diluted to 1: 300 in PBS-T+1% milk for 1h at RT with agitation. The blot was washed as above and developed with exposure time of 2.5 minutes.

Courtesy of Dr. Marcus Miethke, Department of Chemistry & Biochemistry, UCLA

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