FNR | Ferredoxin-NADP+-oxidoreductase

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AS15 2909 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii, Chlorella sorokiniana, Synechococcus PCC 6803


28 st
Item No:
AS15 2909

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product information
Background FNR (ferredoxin-NADP+-oxidoreductase) catalyzes reduction of NADP+ using Fd that has accepted electrons from photosystem II in the final step of linear photosynthetic electron transport. In higher plants FNRs are encoded by a small multiple gene family (two chloroplast-targeted FNR in Arabidopsis and rice and three isoenzymes in maize). All forms are evenly distributed between the thylakoids and stroma.
Immunogen Purified, native FNR from Chlamydomonas reinhardtii
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications western blot (WB)
Related products

AS10 1625 | Anti-FNR | Ferredoxin-NADP+-oxidoreductase (higher plants), rabbit antibodies

other antibodies to proteins involved in photosynthetic electron transfer

Secondary antibodies

Additional information
application information
Recommended dilution 1: 1000 - 3000 with ECL (WB)
Expected | apparent MW

35 kDa

Confirmed reactivity

Chlamydomonas reinhardtii, Chlorella sorokiniana, Synechococcus PCC 6803

Predicted reactivity Higher plants
Not reactive in
Additional information For detection in Chlorella sorokiniana, Synechococcus PCC 6803 higher load per well needs to be applied.
Selected references to be added when available, antibody release in March 2016. 

application example

western blot using anti-FNR antibodies

 10 ng of recombinant Chlamydomonas reinhardtii FNR (1), thylakoid preparation (1 µg chl) from Chlamydomonas reinhardtii cc 124 (2), thylakoid preparation (1 µg chl) of Chlorella sorokiniana (3), thylakoid preparation (1 µg chl) of Synechococcus PCC 6803 (4) incubated with urea based sample buffer over night at 25°C were separated on Bolt 4-12 % Bis-Tris Plus gels (Novex, Life Tech) and blotted 1h to nitrocellulose using iBlot Gel Transfer Stacks Nitrocellulose, Mini (Novex, LifeTech). Blots were blocked with iBind (LifeTech) with iBind solution kit (Novex, LifeTech). Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. There is no washing steps using this set up. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602 ) diluted to 1:40 000 in for 1h at RT with agitation. The blot was washed as above and developed for with EZ-ECL (Biological Industries, Israel). Exposure time was 10 min.

Courtesy of M.Sc. Pini Marcu, Tel Aviv University, Israel

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