PC | plastocyanin

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AS06 141  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, B. juncea, H. annuus, N. tabacum, O. sativa, P. sativum, S. oleracea, S. tuberosum, Z. mays |  cellular [compartment marker] of chloroplast thylakoid lumen


55 st
Item No:
AS06 141

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product information

Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.


Purified native plastocyanin from Spinacia oleracea P00289

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunogold (IG)
Related products

AS06 202 | anti-cytc6 marker antibody of thylakoid lumen for Chlamydomonas reinhardtii

Plant protein extraction buffer

Secondary antibodies

Additional information

cellular [compartment marker] of chloroplast thylakoid lumen

This product can be sold containing proclin if requested

application information
Recommended dilution

1:2000 with alkaline phosphatase (WB), 1: 100 (IG)

Expected | apparent MW

10 kDa

Confirmed reactivity

Arabidops thaliana, Brassica juncea, Heliantus annuus, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Solanum tuberosum, Zea mays

Predicted reactivity

dicots Ricinus communis, Solanum lycopersicum, monocots including Hordeum vulgare, moss Physcomitrella patens

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.

PC1 runs closer to 14 kDa while PC2  runs closer to 19 kDa.  For good resolution adding fresh DTT to the sample buffer is recommended.
PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.

Selected references Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.
Sook Seok et al. (2013). AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability. Physiologia Plantarum, DOI: 10.1111/ppl.12116.
Perera-Garcia et al. (2013). Arabidopsis copper transport protein COPT2 participates in the crosstalk between iron deficiency responses and low phosphate signaling.


application example

western blot image using anti-plastocyanin antibody

10 µg of total protein from (1) Arabidopsis thaliana, (2) Brassica juncea, (3) Zea mays, (4) Oryza sativa, (5) Solamum lycopersicum, (6) Nicotiana tabacum, (7) Heliantus annuus were separated on  SDS-PAGE and blotted to nitrocellulose. Filters were probed with anti-PC antibody  (AS06 141, 1:2000). Signal was developed using alkaline phosphatase conjugated secondary antibody. Each sample was run in duplicate. Signal was developed using alkaline conjugated secondary antibody.


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