PSA2 | Photosystem I assembly factor 2

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AS13 2654 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


59 st
Item No:
AS13 2654

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product information
Background PSA2 (Photosystem I assembly factor 2) is a protein coded by a gene belonging to the "Green Cut" set, found only in green algae and plants but not in non-photosynthetic organisms. PSA2 is localized in thylakoid lumen.
Immunogen recombinant protein corresponding to amino acids 87 to 186 of Arabidopsis thaliana PSA2, UniProt:O64750, TAIR: AT2G34860
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunoprecipitation (IP)
Related products

collection of antibodies to Green Cut proteins

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

20 kDa | 10 kDa (processed form)

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brassica rapa
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references Fristedt et al. (2014). A Thylakoid Membrane Protein Harboring a DnaJ-type Zinc Finger Domain is Required for Photosystem I Accumulation in Plants. J Biol Chem. 2014 Sep 16. pii: jbc.M114.587758.

application example

western blot using anti-PSA2 antibodies

Respective amounts of a leaf total cell extract from Arabidopsis thaliana loaded on total chlorophyll µg/µl were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 10 % milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 seconds.

Courtesty of Dr. Rikard Fristedt, Biophysics of Photosynthesis, Dep. Physics and Astronomy, Faculty of Sciences. VU University of Amsterdam, The Netherlands

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