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CGL160 | Conserved in green lineage 160

265 €
Buy 2 items of this product for 198 €/each
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AS12 1853 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

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Item No:
AS12 1853

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product information
Background

CGL160 (Conserved in green lineage 160) is a protein encoded in the nucleus and localized in chloroplast. It interacts with components of the CF1 sub-complex. Synonymes: ATCGL160.

Immunogen

part of Arabidopsis thaliana recombinant CGL160 derived from a following sequence UniProt: O82279,
TAIR: At2g31040

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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collection of antibodies to GreenCut proteins

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 5000 with standard ECL (WB)

Expected | apparent MW

38.6 | 34 kDa (without transit peptide)

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity please inquire
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references Fristedt et al. (2015). The Thylakoid Membrane Protein CGL160 Supports CF1CF0 ATP Synthase Accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.

application example

western blot using anti-CGL160 antibodies
Respective amounts of
µg/ul of chlorophyll from Arabidopsis thaliana leaf extracted with sample buffer (2% SDS, 8% sucrose, 0.2mM EDTA, 10mM Tris HCl (pH 6.8) 4% beta-mercaptoethanol) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 10 % milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL West Pico (34080, Thermo) according to the manufacturer's instructions. Exposure time was 60 seconds.

Courtesy of Dr. Rikard Fristedt, Biophysics of Photosynthesis, Dep. Physics and Astronomy, Faculty of Sciences. VU University of Amsterdam, The Netherlands


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