STT7 | Serine/threonine-protein kinase STT7, chloroplastic

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AS15 3080 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii


9 st
Item No:
AS15 3080

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product information
Background Serine/threonine-protein kinase stt7, chloroplastic is required for state transition by phosphorylating light-harvesting complex II outer antennae (LCHII).
Immunogen Recombinant STT7 of Chlamydomonas reinhardtii, overexpressed in E.coli, UniProt: Q84V18
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications western blot (WB)
Related products

AS10 1601 | anti-STN8 kinase | serine/threonine-protein kinase STN8, chloroplastic for Arabidopsis thaliana, rabbit antibodies

AS10 1611 | anti-STN7 | serine/threonine-protein kinase STN7, chloroplastic for Arabidopsis thaliana, rabbit antibodies

Secondary antibodies

Additional information
application information
Recommended dilution 1: 1000 - 1: 2 500 (WB)
Expected | apparent MW

80.7 | 80 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity Chlamydomonas reinhardtii
Not reactive in no confirmed exceptions from predicted reactivity are currently known
Additional information to be added when available
Selected references Lameille et al. (2009).  Analysis of the chloroplast protein kinase Stt7 during state transitions. PLoS Biol. 2009 Mar 3;7(3):e45. doi: 10.1371/journal.pbio.1000045.

application example

western blot using anti-STT7 antibodies

Total proteins from Arabidopsis thaliana leaves, corresponding to 1 µg of chlorophylls, were  extracted with loading buffer (10% glycerol, 62.5 mM Tris pH 6.8, 2% SDS, 5% β-mercaptoethanol) and denatured at 100°C (boiling water) for 1 min. Proteins were separated on 12% SDS-PAGE (Laemly) and blotted 1h to PVDF using tank transfer. Blots were blocked with blocking solution (PBS 1X, 0.2% w/v Tween, 5% powder milk) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody diluted in blocking solution, at a dilution of 1: 2 500 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in blocking solution at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG alkaline phosphatase conjugated) diluted to 1:30 000 in blocking buffer  for 1h at RT with agitation. The blot was washed 2 times for 10 min in blocking solution and once with PBS 1X solution for 10 min, then developed in developing buffer developing buffer NBT/BCIP by manual agitation. 

Courtesy of Stefano Cazzaniga, University of Verona, Italy

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