Lhca3 | PSI type III chlorophyll a/b-binding protein

323 €

AS01 007  |  clonality: polyclonal  |  host: rabbit  |  reactivity: monocots and dicots including: A.thaliana, H.vulgare, O. sativa, P. patens, P. sativum, P. vulgaris, S.oleracea


23 st
Item No:
AS01 007

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product information

The light-harvesting protein Lhca3 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca3 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca3 forms a heterodimer (LHCI-680) with Lhca2 that associates with the PSI core close to PsaF and PsaK.
A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.


BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence from Arabidopsis thaliana UniProt: Q9SY97, TAIR: At1g61520. This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms.

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Available antibodies against pigment-binding proteins- LHC

recommended secondary antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 2000 - 1: 5000, detected with standard ECL (WB)

Expected | apparent MW

29 | 25 kDa for Arabidopsis thaliana (due to a transit peptide being cleaved off)

Confirmed reactivity

Arabidopsis thaliana, Aracis hypogae, Bryopsis corticulans, Hordeum vulgare, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Spinacia oleracea, Zea mays

Predicted reactivity

angiosperms (monocots and dicots), gymnosperms

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information Protein is processed into mature form (Jansson 1999).
Selected references Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.
Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.

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