Lhca4 | PSI type IV chlorophyll a/b-binding protein

323 €

AS01 008  |  clonality: polyclonal  |  host: rabbit  |  reactivity: monocots and dicots including A.thaliana, H.vulgare, Nicotiana tabacum, O. sativa, P. patens, S.oleracea, Z.mays


19 st
Item No:
AS01 008

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product information

The light-harvesting protein Lhca4 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca4 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca4 forms a heterodimer (LHCI-730) with Lhca1 that associates with the PSI core close to PsaG and PsaF.
A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.


BSA-conjugated synthetic peptide derived from the Lhca4 protein ofArabidopsis thaliana UniProt: P27521, TAIR: At3g47470. This sequence is highly conserved in Lhca4 proteins of angiosperms (monocots and dicots) and gymnosperms.

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodies

AS01 011 Chlamydomonas | A set of anti-Lhc antibodies for Chlamydmonas

Available antibodies against pigment-binding proteins- LHC

recommended secondary antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 2000 - 1: 5000, detected with standard ECL (WB)

Expected | apparent MW

27.7 | 21 kDa for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana, Bryopsis corticulans, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Spinacia oleracea, Zea mays

Predicted reactivity

angiosperms (monocots and dicots), gymnosperms

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information Protein is processed into mature form (Jansson 1999).
Selected references Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.
Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.

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