Lhcb1 | LHCII type I chlorophyll a/b-binding protein (100 ĩg)
AS01 004-100 | clonality: polyclonal | host: rabbit | reactivity: photosynthetic eukaryotes including A. thaliana, A. hypogaea, C. quitensis Kunt Bartl, H. vulgare, L. esculentum (Solanum lycopersicon), M. crystallinum, N. tabacum, O. sativa, P. sativum, P. vulgaris, S. vulgaris, S. oleracea, Z. mays
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1 : 2000, detected with standard ECL (WB)
|Expected | apparent MW||
25 | 25 kDa for Arabidopsis thaliana
Arabidopsis thaliana, Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Silene vulgaris, Spinacia oleracea, Zea mays
Triticum aestivum, Vitis vinifera, angiosperms (monocots and dicots), gymnosperms, mosses
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
|Additional information||Protein is processed into mature form (Jansson 1999).|
|Selected references||Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.
10 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Hordeum vulgare leaf, (3) Zea mays leaf, (4) Chlamydomonas reinhardtii total cell, (5) Spinacia oleracea total leaf, (6) Physcomitrella patens, (7) Solanum tuberosum total leaf, (8) Solanum esculentum total leaf, all extracted with Protein Extraction Buffer PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2-2.5 % RPN2125 (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:25 000 in TBS-T for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with TMA-6 (Lumigen) detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 2 minutes.
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