Lhcb1 | LHCII type I chlorophyll a/b-binding protein

323 €

AS09 522 |  clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana, Pinus strobus


37 st
Item No:
AS09 522

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product information

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.


BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430  (Lhcb1.4), and At2g34420 (Lhcb1.5)

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 100 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS01 002 Lhcb3 | LHCII type III chlorophyll a/b-binding protein

AS01 003 Lhcb2 | LHCII type II chlorophyll a/b-binding protein

LHC available antibodies against pigment-binding proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII:  Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476

application information
Recommended dilution

1 : 2500 - 1: 5000 with standard ECL (WB)

Expected | apparent MW

28 | 25 kDa for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Pinus strobus

Predicted reactivity

mainly dicots and some monocots, mosses, green algae

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This Lhcb1 antibody is directed specifically against the Arabidopsis Lhcb1 gene products, for those that would prefer higher specific activity over broader specificity offered by Agrisera older Lhcb1 antibody, AS01 004

Protein is processed into mature form (Jansson 1999).

Selected references Correa-Galvis et al. (2016). PsbS interactions involved in the activation of energy dissipation in Arabidopsis. Nature Plants 2, Article number: 15225 (2016) doi:10.1038/nplants.2015.225
Longoni et al. (2015). Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions. Plant Physiol. 2015 Oct 5. pii: pp.01498.2015.
Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.

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