Lhcb1 | LHCII type I chlorophyll a/b-binding protein

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AS01 004 | clonality: polyclonal | host: rabbit  | reactivity: photosynthetic eukaryotes including A. thaliana, A. hypogaea, C. quitensis Kunt Bartl, C. pumilum, H. vulgare, L. esculentum (Solanum lycopersicon), M. crystallinum, N. tabacum, O. sativa, P. sativum, P. vulgaris, R. discolor, S. vulgaris, S. oleracea, Z. mays


46 st
Item No:
AS01 004

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product information

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.
A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII:  Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476


BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb1 proteins from angiosperms (monocots and dicots) and gymnosperms, includingArabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430  (Lhcb1.4), and At2g34420 (Lhcb1.5)

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS01 004 | Lhcb1 | LHCII type I chlorophyll a/b-binding protein

AS01 004PRE | Lhcb1 | LHCII type I chlorophyll a/b-binding protein, pre-immune serum

AS01 011 | 2 | Set of 10 plant anti-Lhca and anti-Lhcb antibodies

AS01 011 CHLAMYDOMONAS | 2 | Set of 4 Chlamydomonas anti-Lhc antibodies

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 2000, detected with standard ECL (WB)

Expected | apparent MW

28 | 25 kDa for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana, Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Rhoeo discolor, Silene vulgaris, Spinacia oleracea, Zea mays

Predicted reactivity

Triticum aestivum, Vitis vinifera, Brachypodium distachyon, Solanum tuberosum, Lotus japonicus, Cucumis sativus, Hordeum vulgare, Nicotiana tabacum, Aegilops tauschii,  angiosperms (monocots and dicots), gymnosperms, mosses

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information Protein is processed into mature form (Jansson 1999).
Selected references Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015.
Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.
Charuvi et al. (2015). Photoprotection Conferred by Changes in Photosynthetic Protein Levels and Organization during Dehydration of a Homoiochlorophyllous Resurrection Plant. Plant Physiol. 2015 Apr;167(4):1554-65. doi: 10.1104/pp.114.255794.
et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.

Application example

western blot using anti-Lhcb1 antibody

10 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Hordeum vulgare leaf, (3) Zea mays leaf, (4) Chlamydomonas reinhardtii total cell, (5) Spinacia oleracea total leaf, (6) Physcomitrella patens, (7) Solanum tuberosum total leaf, (8) Solanum esculentum total leaf, all extracted with Protein Extraction Buffer PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2-2.5 % RPN2125 (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:25 000 in TBS-T for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with TMA-6 (Lumigen) detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 2 minutes.

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