Lhcb1-P | LHCII type I chlorophyll a/b-binding protein, phopshorylated

323 €

AS13 2704  | clonality: polyclonal  |  host: rabbit  |  reactivity:Arabidopsis thaliana


7 st
Item No:
AS13 2704

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product information

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.


KLH-conjugated synthetic peptide RKT*VAKPKGP, where T* indicates phospho-Thr

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 25 ĩg
Reconstitution For reconstitution add 25 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS01 004 | Lhcb1 | LHCII type I chlorophyll a/b-binding protein , rabbit antibodies

AS13 2705 | Lhcb2 | LHCII type II chlorophyll a/b-binding protein, phosphorylated, rabbit antibodies

All anti-LHC antibodies

Antibodies to other proteins involved in photosynthesis

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 10 000 with standard ECL (WB)

Expected | apparent MW

25 | 25 kDa for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Silene vulgaris, Spinacia oleracea, Zea mays
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references Longoni et al. (2015). Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions. Plant Physiol. 2015 Oct 5. pii: pp.01498.2015.
Sato et al. (2015). Chlorophyll b degradation by chlorophyll b reductase under high-light conditions. Photosynth Res. 2015 Apr 21.
Jia et al. (2014). Accumulation of NON-YELLOW COLORING 1 protein of the chlorophyll cycle requires chlorophyll b in Arabidopsis thaliana. Plant J. 2014 Dec 30. doi: 10.1111/tpj.12753.
Leoni et al. (2013). Very rapid phosphorylation kinetics suggest a unique role for Lhcb2 during state transitions in Arabidopsis. Plant J. Oct;76(2):236-46. doi: 10.1111/tpj.12297. Epub 2013 Aug 26.

application example

western blot using anti- phosphorylated Lhcb1 antibody

1 ug of thylakoid membranes isolated from Arabidopsis thaliana wilde-type and mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 1 µg of total chlorophyll was loaded and separated on 16% SDS-PAGE, and then blotted for 2 h onto nitrocellulose membrane. Blots were blocked with milk powder for 2 h and then incubated in the primary antibody solution (AS01 004, 1: 5 000) for 2.5 h, which was then decanted and the blot was washed 3 times for 5 min in TBST. Membrane was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h, followed by washing steps as above. All the steps fallowing transfer were performed in room temperature (RT) with agitation. Membrane was developed for 5 min with ECL according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 30 s increment time for around 5 min.

Courtesy of a phd candidate Małgorzata Pietrzykowska, Umeå Plant Science Centre, Sweden

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