Lhcb5 | CP26 (Lhcb5) homolog, Chlamydomonas

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AS09 407 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii


27 st
Item No:
AS09 407

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product information

Lhcb5 is one of the 3 minor, highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center.


KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence (Q9FEK6).

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 117  Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas

AS09 408 | anti-Lhcbm5 rabbit antibody

Collection of antibodies to Chlamydomonas proteins

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 5000 - 1: 10 000 with ECL (WB)

Expected | apparent MW

30 | 26 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlamydomonas reinhardtii

Not reactive in

other algae

Additional information

for western blot detection image please refer to the article below

Selected references Muranaka et al. (2015). TEF30 interacts with photosystem II monomers and is involved in the repair of photodamaged photosystem II in Chlamydomonas reinhardtii. Plant Physiol. 2015 Dec 7. pii: pp.01458.2015.
Drop et. al (2014). Consequences of state transitions on the structural and functional organization of Photosystem I in the green alga Chlamydomonas reinhardtii. Plant J. 2014 Feb 8. doi: 10.1111/tpj.12459.
Takahashi et al. (2006). Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii. PNAS 103:477-482

application example

western blot using anti-Chlamydomonas Lhcb5 antibodies

Chlamydomonas reinhardtii
membrane extract (A), Chlamydomonas reinhardtii total cell extract, prepared by sonication, loading 14 µl equivalent to 30 µg of total protein (B), Chlamydomonas reinhardtii total cell extract, rapid, prepared directly by spinning down the cells and lysis of cell pellet in SDS-PAGE sample buffer and loading 14 µl equivalent to 98 µg of total protein (C), denatured at 100°C for 5 min. were separated on 15 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 1 % blocking buffer (2 ml blocking reagent stock solution ROCHE 11 520 709 001 in 20 ml TBS) for ON at 4°C without agitation. Blot was incubated in the primary antibody at a dilution of 1: 25 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed according to manufacture instructions. Exposure time was 8 seconds.

Courtesy of Nadine Coosemans, Laboratoire de génétique et physiologie des microalgues, Université de Liège, Belgium

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