aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)

345 €

AS09 580  clonality: polyclonal  |  host: rabbit  |  reactivity: Nicotiana tabacum, Chlamydomonas reinhardtii


4 st
Item No:
AS09 580

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product information

aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection. 


KLH-conjugated peptide derived from known aadA1 sequences

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
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Additional information
application information
Recommended dilution

1: 3 000 with standard ECL (WB)

Expected | apparent MW

30 | 30 kDa

Confirmed reactivity

aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii

Predicted reactivity

aadA cassette

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

to be added when available, antibody available in July 2010


application example 

Solubilized whole  Chlamydomonas reinhardtii cells (1,2) (corresponding to 10 ug  of chlorophyll) and soluble extracts of Nicotiana tabaccum leaves (3,4) were separated on a  15% acrylamide gel containing 8M urea, transferred on nitrocellulose membrane, incubated with anti-aad1 antibodies in 1: 5000 dilution for 1 h in RT, followed by washes and incubation with a secondary anti-rabbuit IgG antibody in  1: 10 000 dilution) and revealed by ECL (GE Healthcare; 3 min exposure). Tested Chlamydomonas reinhardtii and Nicotiana tabaccum strains expressed (+) or not (-) the aadA cassette.
AadA protein in Chlamydomonas reinhardtii migrates more slowly than its Nicotiana tabaccum counterpart as it also includes the 27 first residues of the atpA coding sequence.

Courtesy of Drs Y.Choquet, K. Wostrikoff and D. Drapier, CNRS, France


western blot using aadA1 antibodies

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