FOX1 | plasma membrane ferroxidase FLP1

345 €

AS06 120  | clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii 


14 st
Item No:
AS06 120

Info: Product suggestions Read reviews
product information

A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin; its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.


residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunofluorescence (IF)
Related products

AS07 260 | anti-H+ATPase, plasma membrane rabbit marker antibody

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information

FOX1 is 10-20-fold induced in iron-deficiency and its occurrence therefore serves as a marker of iron nutrition state in Chlamydomonas. For detection image please refer to the article below.

application information
Recommended dilution

1: 1000 - 1: 5000 with standard ECL (WB),

Expected | apparent MW

122 | 120 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlamydomonas reinhardtii

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.

Selected references Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.
LaFontaine et al. (2002) Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii. Eukaryotic Cell  1(5):736-757

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at