CAH3 | Carbonic anhydrase
AS05 073 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii
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1:2000 with standard ECL (WB), 1: 200 (IF)
|Expected | apparent MW||
33.4 | 29 kDa
Chlamydomonas reinhardtii only
|Not reactive in||
Arabidopsis thaliana, Oryza sativa
to be added when available
|Selected references||Mitchell et al. (2014). Dynamics of carbon concentrating mechanism induction and protein re-localisation during the dark to light transition in synchronised Chlamydomonas. Plant Physiol. 2014 Aug 8. pii: pp.114.246918.
Tirumani et al. (2014). Regulation of CCM genes in Chlamydomonas reinhardtii during conditions of light-dark cycles in synchronous cultures. Plant Mol Biol. 2014 Mar 4.
Shutova et al. (2008). The photosystem II-associated Cah3 in Chlamydomonas enhances the O2
evolution rate by proton removal. EMBO J. 27:782-791.
20 µg/ml of chlorophyll from Chlamydomonas reinhardtii Cah3-less mutant (1), and 15 µg/ml of chlorophyll from Chlamydomonas reinhardtii wild-type (2),extracted with 4x Laemmli were separated on 12 % SDS-PAGE and blotted 2h to nitrocellulose filter using tank transfer. Blots were blocked with 5% milk powder for for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 fover night at 8°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed once for 15 min and 3 times for 20 min each in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturer's instructions. Exposure time was 10min. MW standards used, Page ruler, pre-stained from Thermofisher.
Courtesy of Dr. Göran Samuelsson, Umeå Plant Science Centre, Sweden
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