CURT1A | Curvature thylakoid 1A

339 €

AS08 316  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana


6 st
Item No:
AS08 316

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product information

Curvature thylakoid 1A (CURT1A) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D.  It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CURT1A sequence, TAIR: AT4G01150

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), 2D BN/SDS-PAGE
Related products

photosynthetic antibody collection

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

17.6 | 11 kDa (due to N-terminal processing)

Confirmed reactivity

Arabidopsis thaliana, Phaseolus coccineus

Predicted reactivity Nicotiana tabacum
Not reactive in

Hordeum vulgare

Additional information
Selected references

Armbruster et al. (2013). Arabidopsis CURVATURE THYLAKOID1 Proteins Modify Thylakoid Architecture by Inducing Membrane Curvature. 2013 Jul;25(7):2661-78. doi: 10.1105/tpc.113.113118. Epub 2013 Jul 9.

application example

western blot using anti-Curt1A antibodies

The dillution series of  Hordeum vulgare (4; 2; 1 µg Chl) and Arabidopsis thaliana (4; 2; 1 µg Chl) thylakois were separated on 12% Criterion XT Bis-Tris SDS-PAGE (BioRad) gels and blotted for 25min 100V to PVDF membrane. Blot was blocked with 5% fat free skimmed milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 overnight with agitation in 4˚C. The antibody solution was decanted and the blot was washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (swine anti-rabbit IgG horse radish peroxidase conjugated, from Dako) diluted to 1: 5000 in 1% fat free skimmed milk in PBS-T for 1h at RT with agitation. The blot was washed 5 min in PBS-T and 1 min in PBS developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 min.

Courtesy of Dr. Marta Powikrowska, University of Copenhagen, Danmark

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