FeSOD | Chloroplastic Fe-dependent superoxide dismutase

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AS06 125  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. maritima, A. thaliana, Ch. reinhardtii, D.salina, O. sativa, S. tuberosum, Z. mays


17 st
Item No:
AS06 125

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product information

Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD


overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 170 | anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase

AS07 219 | anti-CCS | chloroplastic copper chaperone for SOD

AS09 524 | anti-MnSOD | manganese superoxide dismutase

Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1: 1500 to 1:5000 with alkaline phosphatase (WB)

Expected | apparent MW

25 | 22 kDa

Confirmed reactivity

Armeria maritima, Arabidopsis thaliana, Chlamydomonas reinhardtii, Dunaliella salina, Oryza sativa, Solanum tuberosum, Zea mays

Predicted reactivity

dicots including: Glycine max, Solanum lycopersicum, trees: Pinus pinaster, Populus balsamifera, moss: Physcomitrella patens, algae including: Dunaliella salina, Volvox carteri

Not reactive in


Additional information

The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 μM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251

Selected references Momčilović et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.

Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.

application example


5 µg of stromal protein from (1) Chlamydomonas reinhardtii (left), (2) Arabidopsis thaliana   were separated on  SDS-PAGE. Primary antibodies have been used in 1: 3000.  


Western blot using anti-FeSOD antibodies

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