PsaE | PSI-E subunit of photosystem I

323 €

AS04 047  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Hordeum vulgare, Oryza sativa


19 st
Item No:
AS04 047

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product information

PsaE is a nucleus encoded subunit of the Photosystem I reaction center. It is located on the stroma side and interacts with PsaF. PsaE may be involved in Fd reduction. Alternative name: Photosystem I 10.8 kDa polypeptide


10 kDa PSI-E protein purified from barley thylakoids corresponding to PSI-E protein of Horderum vulgare accession: P13194

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies to PSI proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1:2000 - 1: 5000 with standard ECL (WB)

Expected | apparent MW

10.8 | 10.5 kDa

Confirmed reactivity

Hordeum vulgare, Oryza sativa

Predicted reactivity

Nicotiana sylvestris, Zea mays

Not reactive in

Arabidopsis thaliana, Chlamydomonas reinhardtii, Synechococcus sp. 7942

Additional information

not available at the moment

Selected references

Yadavalli et al. (2012). Differential degradation of photosystem I subunits under iron deficiency in rice. J Plant Physiol. March 22.

Ye et al. (2012). A Mutation of OSOTP 51 Leads to Impairment of Photosystem I Complex Assembly and Serious Photo-damage in Rice. J Integr Plant Biol. Feb 2012.

application example

2 µg of total protein from  (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300),(2) Horderum vulgare leaf extracted with PEB (AS08 300), (3) Chlamydomonas reinhardtii total cell extracted with PEB (AS08 300), (4) Synechococcus sp. 7942 total cell extracted with PEB (AS08 300) were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:20 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).


  Western blot detection using anti-PsaE antibodies

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