PsaF | PSI-F subunit of photosystem I

345 €

AS06 104  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, H. vulgare, N. tabaccum, O. sativa, S. oleracea


13 st
Item No:
AS06 104

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product information

PsaF (PSI-F) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). PsaF has been shown to be involved in the orientation of the soluble electron donor. In plants PSI-F is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.


KLH-conjugated synthetic peptide derived from the PsaF protein sequence of Arabidopsis thaliana (At1g31330). This peptide sequence is not completely conserved in mono- and dicots.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

PSI available antibodies to Photosystem I proteins

Photosynthesis available antibodies to photosynthetic proteins

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1:1000 with standard ECL (WB)

Expected | apparent MW

24 kDa | 17 kDa for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana, Briopsis corticulans, Hordeum vulgare (very weak), Nicotiana tabaccum, Oryza sativa, Spinacia oleracea

Predicted reactivity

Populus trichocarpa, Ricinus communis, Physcomitrella patens, Micromonas sp.

Not reactive in

Chlamydomonas reinhardtii, Synechococcus PCC 7942

Additional information
Selected references Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.


application example

2 µg of total leaf protein of Arabidopsis thaliana (1) and Hordeum vulgare (2) and total cellular protein of Chlamydomonas reinhardtii (3) and Synechococcus PCC 7942 (4) isolated with PEB (AS08 300) were separated on 4-12% Nupage Bis-Tris gels in in MES running buffer (Invitrogen) at 200V for 35 minutes. Proteins were transferred for 80 minutes at 30V to a PVDF membrane pre-wetted in methanol and equilibrated in 1X transfer buffer. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) and probedwith anti-PsaF (AS06 104, 1:1000) and secondary HRP-conjugated goat anti-rabbit antibody (1:50 000, Abcam) for 1 hr in TBS-T containing 2% ECL Advance blocking reagent (GE Healthcare). Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signals was detected after 30 s using ECL Advance detection reagent (GE Healthcare) according to the manufacturers instructions and a CCD imager (FluorSMax, Bio-Rad).

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