PsaH | PSI-H subunit of photosystem I

345 €

AS06 105  |  clonality: polylclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana, Nicotiana tabaccum, Spinacia oleracea


34 st
Item No:
AS06 105

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product information

PsaH (PSI-H) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). Psa-H has been suggested to be involved in regulation of state1-state2 transitions. In plants and algae Psa-H is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.


KLH-conjugated synthetic peptide derived from the protein sequence of Arabidopsis thaliana for PsaH1(At3g16240) and PsaH2 (At1g52230). This peptide sequence is quite conserved in some dicots but not in monocots.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 143 PsaH | PSI-H subunit of photosystem I, Chlamydomonas

PSI available antibodies to Photosystem I proteins

Photosynthesis available antibodies to photosynthetic proteins

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1000 with ECL system (WB)

Expected | apparent MW

10 | 10 for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana, Nicotiana tabaccum, Spinacia oleracea

Predicted reactivity

Arachis hypogaea, Brassica rapa, Medicago truncatula, Nicotiana sylvestris, Nicotiana tabaccum, Populus trichocarpa, Ricinus communis, Spinacia oleracea

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added whan available

Selected references Tiwari et al. (2016). Photodamage of iron–sulphur clusters in photosystem I induces non-photochemical energy dissipation. Nature Plants Article number: 16035 (2016) doi:10.1038/nplants.2016.35.

application example

2 µg of total leaf of Arabidopsis thaliana (1) and Hordeum vulgare (2) and cellular protein of Chlamydomonas reinhardtii (3) and Synechococcus PCC 7942 (4) isolated with PEB (AS08 300) were separated on 4-12% Nupage Bis-Tris gels in in MES running buffer (Invitrogen) at 200V for 35 minutes. Proteins were transferred for 80 minutes at 30V to a PVDF membrane pre-wetted in methanol and equilibrated in 1X transfer buffer. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) and probed with anti-PsaH (AS06 105, 1:1000) and secondary HRP-conjugated goat anti-rabbit antibody (1:50 000, Abcam) for 1 hr in TBS-T containing 2% ECL Advance blocking reagent (GE Healthcare). Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signals was detected after 10 s using ECL Advance detection reagent (GE Healthcare) according to the manufacturers instructions and a CCD imager (FluorSMax, Bio-Rad).


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