PsbA | D1 protein of PSII, N-terminal
AS11 1786 | clonality: polyclonal | host: rabbit | reactivity: higher plants, alage, cyanobacteria
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1:1000 with standard ECL, or 1: 10 000 with ECL Advance (WB)
|Expected | apparent MW||
38 | 28-30 kDa
Arabidopsis thaliana, Hordeum vulgare, Chlamydomonas reinhardtii, Synechococcus sp. 7942
dicots including: Glycine max, Medicago truncatula, Nicotiana tabacum, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Vitis vinifera, and monocots including: Oryza sativa,Triticum aestivum, Zea mays; conifers, brown and red algae, cyanobacteria and diatoms
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.
|Selected references||Malnoë et al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21.
Sook Seok et al. (2013). AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability. Physiologia Plantarum, DOI: 10.1111/ppl.12116.
5 µg of total protein extracted with Protein Extration Buffer, PEB (AS08 300) from (1) Arabidopsis thaliana leaf, (2) Hordeum vulgare leaf, (3) Chlamydomonas reinhardtii total cell, (4) Synechococcus sp. 7942 total cell, were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:25 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).
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