PsbA | D1 protein of PSII, N-terminal

345 €

AS11 1786  | clonality: polyclonal  |  host: rabbit  |  reactivity: higher plants, alage, cyanobacteria


15 st
Item No:
AS11 1786

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product information

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.


KLH-conjugated synthetic peptide derived from N-terminal of available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS01 016 | anti-PsbA C-terminal chicken antibody
AS05 084 | anti-PsbA C-terminal rabbit antibody AS11 1786 | PsbA | D1 protein of PSII, N-terminal, rabbit antibody
AS13 2669 | PsbA | D1 protein of PSII, phosphorylated , rabbit antibody
AS05 084PRE | PsbA | D1 protein of PSII, C-terminal, pre-immune serum
AS10 704 | anti-PsbA | D1 protein of PSII, DE-loop
AS01 016S | PsbA protein standard for quantitation and positive control

Plant protein extraction buffer

Secondary antibodies

Additional information

Peptide target used for antibody production comes from Helix 1 of PSII, lumenal exposed loop. Antibodies are going to recognize the target in a wide range of species.

application information
Recommended dilution

1:1000 with standard ECL, or 1: 10 000 with ECL Advance (WB)

Expected | apparent MW

38 | 28-30 kDa

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare, Chlamydomonas reinhardtii, Synechococcus sp. 7942

Predicted reactivity

dicots including: Glycine max, Medicago truncatulaNicotiana tabacum, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Vitis vinifera,  and monocots including: Oryza sativa,Triticum aestivum, Zea mays; conifers, brown and red algae, cyanobacteria and diatoms

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.

Selected references Malnoë et al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21.
Sook Seok
et al. (2013). AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability. Physiologia Plantarum, DOI: 10.1111/ppl.12116.

application example
western blot detection using anti-PsbA N-terminal antibody

5 µg of total protein extracted with Protein Extration Buffer, PEB (AS08 300) from (1) Arabidopsis thaliana leaf, (2) Hordeum vulgare leaf, (3) Chlamydomonas reinhardtii total cell, (4) Synechococcus sp. 7942 total cell,  were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:25 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

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