PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII

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AS06 142-33  |  clonality: polyclonal  |  host: rabbit  |  reactivity:A.thaliana , F. arundinacea, H.vulgare, S. oleracea, S. tuberosum, P. abies, P. banksiana , C.reinhardtii, Chlorella sp. DT, Synechococcus sp. PCC 7942


17 st
Item No:
AS06 142-33

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product information

PSII reaction centre components are  generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site          of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.


native purified 33 kDa protein from Spinacia oleracea UniProt P12359

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS05 092 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII

AS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII

AS08 305 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII

AS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII

Plant protein extraction buffer

Secondary antibodies

Additional information

total IgG fraction has been purified by 40% ammonium sulpgate precipitation followed by DEAE cellulose chromatography

application information
Recommended dilution

1:2000-1: 5000  with standard ECL (WB)

Expected | apparent MW

35 | 33 kDa

Confirmed reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella sp. DT, Festuca arundinacea cv. Kord, Hordeum spontaneum, Hordeum vulgare, Picea abies, Pinus banksiana, Spinacia oleracea, Solanum tuberosum cultivar Taedong Valley, Synechococcus sp. PCC 7942, Thellungiella salsuginea

Predicted reactivity

dicots including: Nicotiana tabacum, Solanum lycopersicum, trees: Populus trichocarpa

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Selected references Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.
Muranaka et al. (2015). TEF30 interacts with photosystem II monomers and is involved in the repair of photodamaged photosystem II in Chlamydomonas reinhardtii. Plant Physiol. 2015 Dec 7. pii: pp.01458.2015.
Jedmowski et al. (2014). Comparative analysis of drought stress effects on photosynthesis of Eurasian and North African genotypes of wild barley. Photosynthetica, September 2014.
Wiciarz et al. (2014).Enhanced chloroplastic generation of H2 O2 in stress-resistant Thellungiella salsuginea in comparison to Arabidopsis thaliana. Physiol Plant. 2014 Jun 24. doi: 10.1111/ppl.12248.

application example

2 µg of total protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) Hordeum vulgare leaf extracted with PEB (AS08 300), (3) Chlamydomonas reinhardtii total cell extracted with PEB (AS08 300), (4) Synechococcus sp. 7942 total cell extracted with PEB (AS08 300), (5) Anabaena sp. total cell extracted with PEB (AS08 300) were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).


western blot using anti-PsbO antibodies


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