PsbP-like protein (sll1418) | cyanobacterial

345 €

AS10 682  |  clonality: polyclonal  |  host: rabbit  |  reactivity: cyanobacteria


21 st
Item No:
AS10 682

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product information

PsbP-like protein (sll1418) is a cyanobacterial homologue of plant PsbP-like protein. It is localized in the thylakoid membrane and associated with photosystem II. Synonymes: Sll1418 protein


KLH-conjugated synthetic peptide derived from PsbP -like protein of Synechocystis sp. PCC 6803 P73952

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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AS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 2000 with standard ECL (WB)

Expected | apparent MW

20.7 | 17 kDa

Confirmed reactivity

Synechocystis sp. PCC 6803

Predicted reactivity

Anabaena variabilis, Arthrospira maxima, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes, Ostreococcus lucimarinus, Trichodesmium erythraeum,

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

Sveshnikov et al. (2007) The PsbP-like protein (sll1418) of Synechocystis sp. PCC 6803 stabilises the donor side of Photosystem II, Photosynth. Res. 93, 101-109.  Ishikawa et al. . (2005) Functional analysis of the PsbP-like protein (sll1418) in Synechocystis sp. PCC 6803, Photosynth. Res. 84, 257-262.

application example

0.25 µg of chlorophyl a/lane from Synechocystis sp. PCC 6802 wilde type and PsbP-like deletion mutants were separated on 12.5 % SDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PsbP-like antibody (AS10 682, 1:2 000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL detection reagent according the manufacturers instructions (GE Healthcare). Exposure time was 15 seconds.

Courtesy Dr. Sveshnikov


  western blot detection using anti-PsbP-like antibody

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