PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII
AS06 142-23 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana , P. banksiana. , S.oleracea, Ch. reinhardtii
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1:2000 - 1: 5000 with standard ECL (WB)
|Expected | apparent MW||
28 | 23 kDa
Arabidopsis thaliana , Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Chlamydomonas reinhardti
dicots including: Pisum sativum, Solanum lycopersicum, monocots including: Oryza sativa, trees including: Populus balsamifera, Pinus monticola
|Not reactive in||
Synechococcus sp. PCC 7942
Load per well on cell extract of Pinus banksiana (Jack Pine) was 7 µg.
Wang et al. (2008). Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana. Nat Chem Biol. 4: 557-563.
2 µg of total protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) Hordeum vulgare leaf extracted with PEB (AS08 300), (3) Chlamydomonas reinhardtii total cell extracted with PEB (AS08 300), (4) Synechococcus sp. 7942 total cell extracted with PEB (AS08 300), (5) Anabaena sp. total cell extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).
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