PsbS | 22 kDa Lhc-like PSII protein

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AS09 533 | clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, H. vulgare, N. tabacum, P. abies, S. oleracea, Z. mays


47 st
Item No:
AS09 533

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product information

The 22 kDa PsbS protein of photosystem II functions in the regulation of photosynthetic light harvesting. Along with a low thylakoid lumen pH and the presence of de-epoxidized xanthophylls, PsbS is necessary for photoprotective thermal dissipation of excess absorbed light energy in plants, measured as non-photochemical quenching of chlorophyll fluorescence. Synonymes: NPQ4 (NONPHOTOCHEMICAL QUENCHING).


KLH-conjugated synthetic peptide located in solubilized part of the protein, derived from available di and monocot PsbS sequences, including Arabidopsis thaliana (At1g44575)

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS03 032 | anti-PsbS hen antibody

PSII  available antibodies against Photosystem II proteins

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 2000 with standar ECL (WB)

Expected | apparent MW

28 | 22 kDa forArabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Picea abies, Ricinus communis, Spinacia oleracea, Zea mays

Predicted reactivity

dicots including: Cucumis sativus, Medicago truncatulaSolanum lycopersicum, Vitis vinifera, monocots including: Oryza sativa, trees: Picea sitchensis, Pinus radiata, Pinus taeda, Populus balsamifera, moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.
Karlsson et al. (2015). The Arabidopsis thylakoid transporter PHT4;1 influences phosphate availability for ATP synthesis and plant growth. Plant J. 2015 Aug 8. doi: 10.1111/tpj.12962.
Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479.
Belgio et al. (2015). Light harvesting superstructures of green plant chloroplasts lacking photosystems. Plant Cell Environ. 2015 Mar 4. doi: 10.1111/pce.12528.
Lintala et al. (2013). Arabidopsis tic62 trol mutant lacking thylakoid bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype. Mol Plant Sep 16.
et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16.
et al. (2012). LCAA, a novel factor required for Mg protoporphyrin monomethylester cyclase accumulation and feedback-control of aminolevulinic acid biosynthesis in tobacco. Plant Physiol. Oct 19.

application example 1

5 µg of total extract from (1) Arabidopsis thaliana leaf,  (2) Spinacia oleracea  (3) Hordeum vulgare (4) Zea mays extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds. 

western blot using anti-PsbS antibodies


application example 2

western blot using anti-PsbS rabbit antibodies

50 mg of leaf tissue from 3-4 week old Arabidopsis thaliana (columbia) wilde type (1,2) or NPQ4 (3,4) was ground to fine powder in liquid nitrogen with a small plastic grinder and further homogenized in 200 ul of 1x Sample Loading Buffer (12 % glycerol, 60 mM Tris-HCl pH 6.8, 2.2 % SDS, 0.04 % bromophenol blue, 1.2 % beta-mercaptoethanol). Samples were heated at 95C for 1.5 minute and spun 30 seconds at 12k rpm. 10 ul of total protein extract was loaded onto BoltTM 4-12 % Bis-TrisPlusGels (Invitrogen) and run for 40 min. at 165 V and transferred to nitrocellulose in mini Bolt module for 1 h at 10V. Blot was blocked in 5 % nonfat dry milk in TBST (NaCl 137 mM, KCl 2.7 mM, Tri base 19 mM) and incubated with primary antibodies at 1: 1000 dilution for 1 hour at RT. After 4x5 minute wash in TBST, secondary antibody incubation (goat anti-rabbit IgG HRP conjugated, Agrisera AS09 602) at a dilution of 1: 10 000 for 1 h was follwed by 4x5 min. washes. Blots were well drained and incubaed briefly with PierceSuperSignalWest Pico Chemiluminescent Sustraste before exposure to ImageQuant ccd camera.

Courtesy of Dr. Laura Roy, University of Amsterdam, The Netherlands

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