PsbZ | ycf9 protein of PSII

345 €

AS06 115  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana, Pisum sativum


10 st
Item No:
AS06 115

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product information

The 6.5 kDa PSII subunit PsbZ (ycf9 in Chlamydomonas) is coded by an open reading frame that is ubiquitously present in chloroplast and cyanobacterial genomes. PsbZ seems to control the interaction of PSII cores with the light-harvesting antenna involving changes in protein phosphorylation within PSII units, the deepoxidation state of xanthophylls, and the kinetics and amplitude of nonphotochemical quenching (NPQ).


KLH-conjugated synthetic peptide derived from PsbZ protein sequence of Arabidopsis thaliana (AtCg00300). This sequence is highly conserved in dicots, monocots, and Physcomitrella patens but only weakly in Chlamydomonas reinhardtii.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

PSII available antibodies to Photosystem II proteins

Photosynthesis available antibodies to photosynthetic proteins

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

6.5 | 6.5 kDa for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana, Pisum sativum

Predicted reactivity

dicots and monocots, Physcomitrella patens

Not reactive in


Additional information

to be added when available

Selected references

to be added when available [product has been available since 2009-04-02]

application example

5 µg of total protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) chloroplast, (3) thylakoids, (4) chloroplasts from high light treated plants, (5) thylakoids from high light treated plants, (6) PSI preparation , (7) Pisum sativum BBY particles were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PsbZ (AS06 115, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (Invitrogen) using a Fuji LAS-3000 CCD (240s, standard sensitivity). There is a strong cross-reaction between 20-30 kDa.

Western blot detection using PsbZ antibody


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