Cyt c | cytochrome c

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AS08 343A  |  clonality:polyclonal  |  host: rabbit  reactivity: Arabidopsis thaliana, Lupinus luteus| marker of PCD


11 st
Item No:
AS08 343A

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product information

Cytochrome c is located in inner mitochondrial membrane. It is a small heme protein which, unlike other cytochromes, is highly soluble. This protein is an essential component of the electron transport chain, where it undergoes oxidation and reduction without binding oxygen.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana cytochrome c protein sequence, UniProt:D7KMK0 (C-1) D7LY03 (C-2) , TAIR: At1g22840 (Cytc1) and At4g10040 (Cytc2)

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water

store lyophilized at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles and store at -80°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
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AS04 052 | anti-COXII hen antibody

AS04 053A | anti-COXII rabbit antibody

AS06 151 | anti-COXIIb antibody

secondary antibodies

Additional information
application information
Recommended dilution

1: 5000 with standard ECL (WB) 

Expected | apparent MW

12,5 | 14 kDa for A. thaliana

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

cytc1 and cytc2 from following species: A. theoprasi, B. napus, B. oleracea, C. maxima, Ch. reinhardtii (peptide target partially conserved), L. esculentum, Lupinus luteus, M. truncatula, O. sativa, Ostreococcus (peptide target partially conserved), P. aurea, Physcomitrella patens, R. comunis, S. nigra, V. vinifera

Not reactive in

Arabidopsis thaliana CytC6

Additional information

The presence of cytochrome c in the cysotol is a marker of PCD (programmed cell death).

Selected references to be added when available, antibody release in November 2014

application example

western blot using affinity purified anti-cytochrome c antibodies

Mitochondrial proteins (15 ug) from Arabidopsis thaliana mitochondria was separated on 16% acrilamide gel and electrophoresis prepared according to Schägger and von Jagov (Anl. Biochem., 1987, 166:368-379). After running the gel, proteins were transferred to PVDF membrane using wet transfer (Roti®-Blot 2, Roth). Transfer was checked by Ponceau S staining. Blot was destained by several quick washings in distilled water and 1 washing in 1X TBS (10 mM T pH 7.5, 150 mM NaCl) (10-15 min.).Blot was blocked by 1.5 hour in 5% milk in TBST (1X TBS, 0,1% Tween 20) After blocking blot was washed quickly twice in TBST and incubated 2 hours with primary antibody (dilution 1: 1000) in TBST. Washing: two quick washings in TBST and 3 x 10 min. washings in TBST. Then blot was incubated 45-60 min. with a secondary anti-rabbit antibodies conjugated to peroxidase (Agrisera AB, dilution 1:10 000, AS09 602) in TBST. Washing: as above. After washing blot was incubated 1-2 min. in ECL solution (NOWA Kit, Thermo Scientific). Chemiluminescence was detected by BioSpectrum® Imaging System (UVP). Exposure time was 5 seconds.

Courtesy Dr. Janusz Piechota, Wrocław University, Poland

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