Idh | isocitrate dehydrogenase
AS06 203A | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, C.annuum, L. esculentum, P. sativum, S. tuberosum, Z. mays | cellular [compartment marker] of mitochondrial matrix
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1 : 5 000 with standard ECL (WB)
|Expected | apparent MW||
39 | 45 kDa (Arabidopsis thaliana)
Arabidopsis thaliana, Capsicum annuum, Lycopersicum chilense, Nicotiana benthamiana, Solanum lycopersicum , Pisum sativum, Solanum sogarandium, Solanum tuberosum, Zea mays
Brachypodium distachyon, Brassica napus, Capsella rubella, Citrus sinensis, Glycine max, Hordeum vulgare, Malus x domestica, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays
|Not reactive in||
cellular [compartment marker] of mitochondrial matrix
|Selected references||Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.
Choi et al. (2014). Pepper Mitochondrial FORMATE DEHYDROGENASE1 Regulates Cell Death and Defense Responses against Bacterial Pathogens. Plant Physiol. 2014 Sep 18. pii: pp.114.246736.
Barreto et al. (2014). Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response. BMC Plant Biol. 2014 May 28;14(1):144.
Szabala et al. (2014). Accumulation of acidic SK3 dehydrins in phloem cells of cold- and drought-stressed plants of the Solanaceae. Planta, Jan 7.
20 µg of total protein from (1) Arabidopsis thaliana leaf extract, (2) Arabidopsis thalianafraction enriched with mitochondria, (3) Arabidopsis thaliana pure mitochondria, (4) Pisum sativum pure mitochondria, (5) Solanum tuberosum pure mitochondria were separated on 4-12% SDS-PAGE and blotted to nitrocellulose. Blots were blocked immediately following transfer in 5% milk powder in TBS. Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation. Blots were developed using ECL reagent (GE Healthcare).
* Band detected at ca. 90 kDa is suspected to be a dimmer of Idh, since this band is depleted upon peptide competition experiment.
15 µg of total protein stem extract from Lycopersicum esculentum (1), pure mitochondrial fraction isolated from stems of Lycopersicum esculentum (2) , pure mitochondrial fraction isolated from stems of Capsicum annuum (3) , pure mitochondrial fraction isolated from tubers of Solanum tuberosum (4) were separated on 10% SDS-PAGE and blotted onto nitrocellulose . After blocking with 5% milk in TBST , blots were incubated with the primary antibody at a dilution of 1:1000 in TBST for 1.5h at room temperature. Following incubation and wash steps, blots were incubated withSIGMA secondary Anti-Rabbit IgG , Alkaline Phosphatase Conjugate for 1 hour at a dilution of 1:40000 . Blots were developed with the alkaline phosphatase detection system using NBT/BCIP (SIGMA).
Courtesy of Bartosz Szabala, Institute of Plant Genetics, Polish Academy of Science.
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