SHMT | Serine hydroxymethyltransferase

345 €

AS05 075  |  clonality: polyclonal  |  host: rabbit  |  reactivity:  A. thaliana, P. sativum, S. oleracea, V. faba  |  cellular[compartment marker] of mitochondrial matrix


9 st
Item No:
AS05 075

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product information

Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex.  


Purified SHMT protein from Spinacia oleracea

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1:5 000 with standard ECL (WB)

Expected | apparent MW

53 kDa (Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana, Pisum sativum, Spinacia oleracea, Vicia faba

Predicted reactivity

dicots including: Glycine max, Medicago truncatula,  Ricinus communis, Solanum tuberosum, Vitis vinifera, monocots: Hordeum vulgare, trees: Populus balsamifera, algae: Chlamydomonas reinhardii

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

this antibody can be used on total leaf extracts and isolated mitochondria

Selected references Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.
Wei et al. (2013). Folate polyglutamylation eliminates dependence of activity on enzyme concentration in mitochondrial serine hydroxymethyltransferases from Arabidopsis thaliana. Arch Biochem Biophys 1;536(1):87-96.
Camejo et al. (2012). Salinity-induced changes in S-nitrosylation of pea mitochondrial proteins. J. Proteomics Dec.11.

application example



total leaf extract from Arabidopsis thaliana has been loaded per lane based on a leaf disc area. Primary antibody has been used in dilution 1: 5000 following detection using standard ECL. c


western blot detection using anti-SHMT antibody

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