VDAC1 | voltage-dependent anion-selective channel protein 1
AS07 212 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, di and monocots,| cellular [compartment marker] of mitochondrial outer membrane
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1:5000 on 2-30 µg of protein/lane with standard ECL (WB), 1: 500 (IL)
|Expected | apparent MW||
29 kDa (for Arabidopsis thaliana)
Arabidopsis thaliana, Beta vulgaris, Brassica oleracea var. botrytis, Oryza sativa, Papaver sp. pollen tubes (IL), Spinacia oleracea, Physcomitrella patens
Arabidopsis alpina, Aundo donax, Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Citrus sinensis, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium arboreum, Hoedum vulgare var. distichum, Jatropha curcas, Medicago truncatula, Mesembryanthemum crystallinum, Morus notabilis, Nicotiana tabacum, Phaseolus coccineus, Phaseolus vulgaris, Pisum sativum, Plantago major, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays
|Not reactive in||
Chlamydomonas reinhardtii, Glycine max, Zea mays, diatoms, Saccharomyces cerevisiae
Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.
Immunolocalization method description and images are available here
Blue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010
|Selected references||de Michele et al. (2016). Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings. J Proteome Res. 2016 Mar 4;15(3):900-13. doi: 10.1021/acs.jproteome.5b00876. Epub 2016 Feb 4.
Li et al. (2015). A Chaperone Function of NO CATALASE ACTIVITY1 Is Required to Maintain Catalase Activity and for Multiple Stress Responses in Arabidopsis. Plant Cell. 2015 Feb 19. pii: tpc.114.135095.
Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.
Hsueh et al. (2014). The chloroplast outer envelope protein P39 in Arabidopsis thaliana belongs to the Omp85 protein family. Proteins. 2014 Nov 17. doi: 10.1002/prot.24725.
Takahashi et al. (2014). Transport of rice cyclobutane pyrimidine dimer (CPD) photolyase into mitochondria relies on a targeting sequence located in its C-terminal internal region.
Alcántar-Aguirre et al.(2013).ATP produced by oxidative phosphorylation is channeled toward hexokinase bound to mitochondrial porin (VDAC) in beetroots (Beta vulgaris). Planta, March 17.
Arabidopsis thaliana membrane extraction and SDS–PAGE analysis About 200 mg (gFW) Arabidopsis seedlings (3-week-old), grown on 1% MS-agar plates, was ground with mortar and pestle in the presence of 2 ml extraction buffer [75 mM MOPS-KOH, 0.6 M Sucrose, 4 mM EDTA, 0.2% PVP-40, 0.2% BSA, 8 mM L-cystein, pH 7.6] and the protease inhibitor cocktail ‘complete Mini’ from Roche Diagnostics GmbH (Mannheim, Germany). Crude membrane extracts were prepared essentially as described in Colas des Francs-Small et al. (2012). The membranous fraction was obtained by centrifugation at 22,000 g for 10 min at 4oC. The pellet containing the crude membranous fraction was washed twice with wash buffer [37.5 mM MOPS-KOH, 0.3 M Sucrose, 2 mM EDTA pH 7.6]. The samples were kept frozen at -80oC until used. For SDS-PAGE, an aliquot equivalent to 10 mg (i.e. 1x dilution) of crude Arabidopsis membrane extracts was solubilized in 3x Laemmli sample buffer (Bio-Rad) and the proteins were analyzed by SDS-gel electrophoresis.
Courtesy of Dr. Oren Ostersetzer, The Hebrew University of Jerusalem, Israel
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